Systematic Development of a Detergent Toolbox as an Alternative to Triton X-100.

ABSTRACT: Detergents are routinely included in protein purification processes to inactivate enveloped viruses that may arise from adventitious or endogenous contamination. The detergent Triton X-100 (TX-100) has been widely used as part of the production process for therapeutic proteins. However, recent ecological studies indicate that TX-100 and its metabolites detrimentally impact aquatic organisms, thus alternative detergents for viral inactivation are required. The overall aim of this study was to identify one or more detergents that are a suitable replacement for TX-100 in the viral inactivation step. In stage one, 16 potential alternatives were identified and screened against TX-100 using multiple criteria such as solubility, feasibility of virus inactivation, critical micelle concentration, a

SUBMITTER: Yadav V 

PROVIDER: S-EPMC11975162 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature