Effects of Air Temperature on Transient Expression of Influenza Hemagglutinin in Nicotiana benthamiana: Analysis of Transgene Transcription and Plant Stress Responses.

ABSTRACT: Postinfiltration air temperature is known to affect the accumulation of recombinant protein in Agrobacterium-mediated transient expression in Nicotiana benthamiana plants, including the number of days needed to reach maximum content and the rate of reduction thereafter. This study aimed to clarify whether the transcript levels of the transgenes and those of plant stress response markers (i.e., hypersensitive response and endoplasmic reticulum [ER] stress) could be primary determinants of the accumulation of recombinant influenza hemagglutinin (HA) at 21 or 26°C. We found no correlation between the transgene expression levels (HA, RdRP, and MP) and the number of days needed to reach the maximum HA protein content at both temperatures. Regardless of the accumulation compartment, HA protein c

SUBMITTER: Pruksarojanakul P 

PROVIDER: S-EPMC11975175 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature