In vitro Generated Megakaryocytes for the Detection of Human Platelet Antigen-Specific Alloantibodies.

ABSTRACT:

Introduction

Serologic characterization of antihuman platelet antigen (HPA) alloantibodies is crucial in fetal neonatal alloimmune thrombocytopenia. The gold standard MAIPA assay requires fresh platelets from HPA-genotyped donors, which is challenging for some laboratories. Megakaryocytes express HPA epitopes and offer an alternative source for detecting anti-HPA antibodies. The objective of this study was to assess the efficacy of a novel assay called monoclonal antibody immobilization of megakaryocyte antigens (MAIMA) for detecting anti-HPA antibodies.

Methods

CD34+ cells from buffy coats were differentiated into megakaryocytes in vitro. The performance of the MAIMA assay was evaluated using WHO reference reagents for HPA-1a, HPA-3a, and HPA-5b, along with sera samples fro

SUBMITTER: Uzun G 

PROVIDER: S-EPMC11975340 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature