Inferring kinase-phosphosite regulation from phosphoproteome-enriched cancer multi-omics datasets.

ABSTRACT: Phosphorylation in eukaryotic cells plays a key role in regulating cell signaling and disease progression. Despite the ability to detect thousands of phosphosites in a single experiment using high-throughput technologies, the kinases responsible for regulating these sites are largely unidentified. To solve this, we collected the quantitative data at the transcriptional, protein, and phosphorylation levels of 10 159 samples from 23 tumor datasets and 15 adjacent normal tissue datasets. Our analysis aimed to uncover the potential impact and linkage of kinase-phosphosite (KPS) pairs through experimental evidence in publications and prediction tools commonly used. We discovered that both experimentally validated and tool-predicted KPS pairs were enriched in groups where there is a significant

SUBMITTER: Cheng H 

PROVIDER: S-EPMC11975364 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature