A single-cell atlas of spatial and temporal gene expression in the mouse cranial neural plate.

ABSTRACT: The formation of the mammalian brain requires regionalization and morphogenesis of the cranial neural plate, which transforms from an epithelial sheet into a closed tube that provides the structural foundation for neural patterning and circuit formation. Sonic hedgehog (SHH) signaling is important for cranial neural plate patterning and closure, but the transcriptional changes that give rise to the spatially regulated cell fates and behaviors that build the cranial neural tube have not been systematically analyzed. Here, we used single-cell RNA sequencing to generate an atlas of gene expression at six consecutive stages of cranial neural tube closure in the mouse embryo. Ordering transcriptional profiles relative to the major axes of gene expression predicted spatially regulated expression

SUBMITTER: Brooks ER 

PROVIDER: S-EPMC11975377 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature