Unravelling the Programmed Inflammation and Tissue Repair by a Multipotential Antimicrobial K21 Silane.

ABSTRACT:

Aims and objectives

To examine if a novel antimicrobial silane K21 can alter macrophage polarisation and affect fibroblast proliferation by deciphering the molecular pathways for programmed healing using a combined in vitro and in vivo (animal) burn model.

Materials and methods

An injectable silane-based antimicrobial aimed to modulate macrophage polarisation was manufactured. Experimental analysis included colorimetric cell migration assays on gingival fibroblasts, macrophage phagocytosis characterisation, immunofluorescence staining, triacylglycerol accumulation within macrophages by LCMS, cellular metabolic/proliferation assays, macrophage exposure quantification with morphology assessment using FE-SEM, Raman spectral analysis, RNA isolation for relative gene expression a

SUBMITTER: Daood U 

PROVIDER: S-EPMC11976552 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature