Single-nucleus transcriptional and chromatin accessible profiles reveal critical cell types and molecular architecture underlying chicken sex determination.

ABSTRACT:

Introduction

Understanding the sex determination mechanisms in birds has great significance for the biological sciences and production in the poultry industry. Sex determination in chickens is a complex process that involves fate decisions of supporting cells such as granulosa or Sertoli cells. However, a systematic understanding of the genetic regulation and cell commitment process underlying sex determination in chickens is still lacking.

Objectives

We aimed to dissect the molecular characteristics associated with sex determination in the gonads of chicken embryos.

Methods

Single-nucleus RNA-seq (snRNA-seq) and ATAC-seq (snATAC-seq) analysis were conducted on the gonads of female and male chickens at embryonic day 3.5 (E3.5), E4.5, and E5.5.

Results

Here, we

SUBMITTER: Li J 

PROVIDER: S-EPMC11976570 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature