Circ_0003072 Mediates the Pro-osteogenic Differentiation Effect of Betulinic Acid on Human Periodontal Ligament Stem Cells.

ABSTRACT:

Background

Betulinic acid (BetA) exhibits a good pro-osteogenic differentiation effect on human periodontal ligament stem cells (hPDLSCs), making it a promising supplement for periodontal regeneration. Circular RNAs (circRNAs) have emerged as important regulators of cellular behaviour, and whether circRNAs are involved in the effects of BetA remains unknown.

Methods

Bioinformatics analysis was used to screen for dysregulated circRNAs involved in osteogenic differentiation based on public datasets. Osteogenic differentiation was evaluated using quantitative PCR detection of RUNX2, ALPL, COL1A1, and BGLAP levels, alkaline phosphatase staining, and Alizarin Red S staining of calcified nodules. The role of circ_0003072 in the BetA-regulated osteogenic differentiation of hPDLSCs

SUBMITTER: Qi Y 

PROVIDER: S-EPMC11976600 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature