Osteogenic Induction Activity of Magnesium Chloride on Human Periodontal Ligament Stem Cells.

ABSTRACT:

Objectives

Periodontal ligament stem cells (PDLSCs) are promising for regenerative therapies due to their self-renewal and multilineage differentiation, essential for periodontal tissue repair. Although magnesium plays a vital role in bone metabolism, its specific effects on PDLSCs and potential applications in regeneration are unclear. This study aimed to investigate the effects of magnesium chloride (MgCl₂) on the proliferation and osteogenic differentiation of human PDLSCs (hPDLSCs).

Methods

hPDLSCs were isolated, characterised, and treated with 0.1-40 mM MgCl₂. Cell viability and proliferation were assessed using an MTT assay. Cell migration was measured by a scratch assay. Colony-forming unit formation and cell cycle analysis were examined using crystal violet and propi

SUBMITTER: Lumbikananda S 

PROVIDER: S-EPMC11976617 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature