Optimization of methods for isolation and purification of outer membrane vesicles (OMVs) from Neisseria lactamica.

ABSTRACT: Outer membrane vesicles (OMVs) are nanoparticles released by Gram-negative bacteria during growth, mainly under stress conditions. OMV-based vaccines have played an important role in vaccination against Neisseria meningitidis serogroup B (MenB), stimulating research into novel approaches for developing more effective vaccines. OMVs released by the bacterium Neisseria lactamica have emerged as a promising platform for new vaccine development, especially as carriers in subunit vaccines. Despite their importance, some challenges remain in obtaining and purifying OMVs. The most commonly employed methods for OMV isolation and purification are ultracentrifugation (UC) and size exclusion chromatography (SEC). However, these techniques could present limitations for large-scale production and often

SUBMITTER: Preto RM 

PROVIDER: S-EPMC11976763 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature