High-Throughput Chemotherapeutic Drug Screening System for Gastric Cancer (Cure-GA).

ABSTRACT:

Background

Three dimensional (3D) cell cultures can be effectively used for drug discovery and development but there are still challenges in their general application to high-throughput screening. In this study, we developed a novel high-throughput chemotherapeutic 3D drug screening system for gastric cancer, named 'Cure-GA', to discover clinically applicable anticancer drugs and predict therapeutic responses.

Methods

Primary cancer cells were isolated from 143 fresh surgical specimens by enzymatic treatment. Cell-Matrigel mixtures were automatically printed onto the micropillar surface then stabilized in an optimal culture medium for 3 days to form tumoroids. These tumoroids were exposed in the drug-containing media for 7 days. Cell viability was measured by fluorescence im

SUBMITTER: Lee J 

PROVIDER: S-EPMC11976768 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature