Region-specific brain decellularized extracellular matrix promotes cell recovery in an in vitro model of stroke.

ABSTRACT: Brain decellularized extracellular matrix (ECM) can be an attractive scaffold capable of mimicking the native ecosystem of the central nervous system tissue. We studied the in vitro response of neural cultures exposed to region-specific brain decellularized ECM scaffolds from three distinct neuroanatomical sections: cortex, cerebellum and remaining areas. First, each brain region was evaluated with the isotropic fractionator method to understand the cellular composition of the different cerebral areas. Second, the cerebral regions were subjected to the decellularization process and their respective characterization using molecular, histological, and ultrastructural techniques. Third, the levels of neurotrophic factors in the decellularized brain scaffold were analyzed. Fourth, we studied t

SUBMITTER: Reginensi D 

PROVIDER: S-EPMC11976941 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature