Obesogenic diet leads to luminal overproduction of the complex IV inhibitor H₂ S and mitochondrial dysfunction in mouse colonocytes.

ABSTRACT: Obesity is characterized by systemic low-grade inflammation associated with disturbances of intestinal homeostasis and microbiota dysbiosis. Mitochondrial metabolism sustains epithelial homeostasis by providing energy to colonic epithelial cells (CEC) but can be altered by dietary modulations of the luminal environment. Our study aimed at evaluating whether the consumption of an obesogenic diet alters the mitochondrial function of CEC in mice. Mice were fed for 22 weeks with a 58% kcal fat diet (diet-induced obesity [DIO] group) or a 10% kcal fat diet (control diet, CTRL). Colonic crypts were isolated to assess mitochondrial function while colonic content was collected to characterize microbiota and metabolites. DIO mice developed obesity, intestinal hyperpermeability, and increased endoto

SUBMITTER: Guerbette T 

PROVIDER: S-EPMC11977527 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature