Genetic determinants of HIV-1 subtype C Nef-mediated SERINC3 down-regulation.

ABSTRACT:

Background

Nef-mediated down-regulation of the host restriction factors SERINC3 and SERINC5 significantly enhances HIV-1 infectivity. Natural Nef polymorphisms that affect SERINC3 down-regulation are not as well-characterised as those that affect SERINC5 down-regulation, particularly in HIV-1 subtype C infection. We therefore aimed to identify genetic determinants of SERINC3 down-regulation by subtype C Nef. In addition, we investigated the role of SERINC3 down-regulation activity in disease progression and its contribution to overall Nef function, using Nef fitness model-derived E values as a proxy for overall Nef function in vivo.

Methods

SERINC3 down-regulation activity of 107 participant-derived Nef clones was measured using a flow cytometry-based assay in a T cell line.

SUBMITTER: Samlall N 

PROVIDER: S-EPMC11977927 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature