Brachyury promotes proliferation and migration of colorectal cancer cells by targeting MMP14.

ABSTRACT:

Background

The incidence and mortality rates of colorectal cancer (CRC) are rising, and it is the second most common cause of cancer-related deaths worldwide. Although the transcription factor, Brachyury is intricately linked with various clinical malignancies, the mechanisms by which it influences CRC cell proliferation and migration are inadequately understood.

Methods

Tissue microarray was used to evaluate Brachyury expression in CRC and adjacent normal tissues. The effects of Brachyury on HCT116 and SW480 CRC cells were also examined in vitro, including using Cell Counting Kit-8, colony formation, and transwell assays, and in vivo through subcutaneous tumorigenesis assays in a nude mouse xenograft model. Chromatin immunoprecipitation was used to evaluate Brachyury bindin

SUBMITTER: Chen M 

PROVIDER: S-EPMC11977941 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature