Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencing.

ABSTRACT: 5-Methylcytosine (m⁵C) is one of the posttranscriptional modifications in mRNA and is involved in the pathogenesis of various diseases. However, the capacity of existing assays for accurately and comprehensively transcriptome-wide m⁵C mapping still needs improvement. Here, we develop a detection method named DRAM (deaminase and reader protein assisted RNA methylation analysis), in which deaminases (APOBEC1 and TadA-8e) are fused with m⁵C reader proteins (ALYREF and YBX1) to identify the m⁵C sites through deamination events neighboring the methylation sites. This antibody-free and bisulfite-free approach provides transcriptome-wide editing regions which are highly overlapped with the publicly available bisulfite-sequencing (BS-seq) datasets and al

SUBMITTER: Zhou J 

PROVIDER: S-EPMC11978299 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature