Dynamics of compartment-specific proteomic landscapes of hepatotoxic and cholestatic models of liver fibrosis.

ABSTRACT: Accumulation of extracellular matrix (ECM) in liver fibrosis is associated with changes in protein abundance and composition depending upon etiology of the underlying liver disease. Current efforts to unravel etiology-specific mechanisms and pharmacological targets rely on several models of experimental fibrosis. Here, we characterize and compare dynamics of hepatic proteome remodeling during fibrosis development and spontaneous healing in experimental mouse models of hepatotoxic (carbon tetrachloride [CCl₄] intoxication) and cholestatic (3,5-diethoxycarbonyl-1,4-dihydrocollidine [DDC] feeding) injury. Using detergent-based tissue extraction and mass spectrometry, we identified compartment-specific changes in the liver proteome with detailed attention to ECM composition and chan

SUBMITTER: Jirouskova M 

PROVIDER: S-EPMC11978302 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature