Refining the annotation of <i>Rhodnius prolixus</i> aspartic proteases A1 family genes through proteogenomics.

ABSTRACT: Rhodnius prolixus Stål (Hemiptera: Reduviidae: Triatominae) a hematophagous model organism and vector of Chagas disease, relies on a complex repertoire of digestive enzymes to process its blood meals. Among these, aspartic proteases from the A1 peptidase family play a crucial role in nutrient breakdown. This study aims to refine the gene annotation of the A1 peptidase family in this organism through proteogenomics. A comprehensive analysis of aspartic protease gene sequences and protein isoforms, identified by proteomics, revealed discrepancies in existing gene annotations, including the identification of novel open reading frames and the consolidation of previously separated gene sequences. Our efforts led to the correction of seven gene annotations, reducing the total count of A1

SUBMITTER: Ouali R 

PROVIDER: S-EPMC11978366 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature