Early and sensitive diagnosis of celiac autoimmune disease by using carboxylic acid functionalized magnetic nanoparticles-assisted biosensing platform.

ABSTRACT: A novel impedimetric magneto-immunosensor based on iron oxide (Fe₃O₄) nanoparticles coated with 3-phosphonopropionic acid (3-PPA) (functionalized magnetic beads, or FMBs) was created for the highly sensitive and selective detection of anti-tissue transglutaminase antibody (anti-tTG) in human serum. This label-less immunosensor was introduced by magnetically attaching FMBs onto the working electrode surface with a neodium magnet. The FMBs were utilized as a sensing interface and had carboxylic acid groups for tTG molecules, which could selectively link the target anti-tTG antibody. The FMBs modification steps were carried out in microcentrifuge tubes and concentrated with magnetic force before electrochemical analyses. The specific immuno-interactions on the FMBs surfa

SUBMITTER: Aydın EB 

PROVIDER: S-EPMC11978692 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature