Characterization of β-glucosidase activity of a Lactiplantibacillus plantarum 6-phospho-β-glucosidase.

ABSTRACT: β-Glucosidases are useful for hydrolysis of glycosidically-bound volatiles (GBV), thereby facilitating the release of aroma chemicals from the fruit matrices. In this study, 10 putative glycosyl hydrolases belonging to GH1 family from Lactiplantibacillus plantarum NCIM 2903 were cloned and recombinantly expressed. Interestingly, only one (LpBgl5) of the nine soluble proteins, previously characterized as a 6-phospho-β-glucosidase showed β-glucosidase activity which was further characterized. The enzyme had an optimum pH and temperature of 6 and 40°C, respectively, and was categorized as aryl-β-glucosidase due to its ability to hydrolyze different natural as well as synthetic glucosides except cellobiose. The enzyme exhibited functional activity across multiple substrates, with relative acti

SUBMITTER: Godse R 

PROVIDER: S-EPMC11978721 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature