Intranasal administration of a panreactive influenza antibody reveals Fc-independent mode of protection.

ABSTRACT: Monoclonal antibodies have two core mechanisms of protection: an antibody's antigen-binding fragment (Fab) can bind and neutralize viral pathogens and its fragment crystallizable domain (Fc) catalyzes effector functions. We investigated the relative contribution of Fab- versus Fc-mediated mechanisms of protection through passive administration of distinct forms of the pan-reactive anti-influenza antibody CR9114. We demonstrated that the contribution of Fc-independent (Fab-dependent) versus Fc-dependent mechanisms of protection is defined by the route of administration. We used CR9114 variants (wild-type, two Fc-silenced variants, or the bivalent antigen-binding fragment F(ab')₂), administered either intravenously or intranasally. We found that intravenously-administered CR9114 r

SUBMITTER: Beukenhorst AL 

PROVIDER: S-EPMC11978755 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature