Enhancing lateral flow immunoassay performance for cardiac troponin I detection with pore-size tailored silica nanoparticles and smartphone-based "AdaptiScan" analysis.

ABSTRACT: The accurate and rapid detection of cardiac troponin I (cTnI) at the point of care is crucial for the timely diagnosis of myocardial infarction (MI). This study introduces an advanced lateral flow immunoassay (LFIA) platform for cTnI detection. We employed small-sized, large-pore dendritic mesoporous silica nanoparticles (DMSN-2) to encapsulate quantum dots (QDs), achieving an enhanced QD loading capacity of 1.427 g QD/g silica, compared to 0.881 g QD/g silica for smaller pore counterparts (DMSN-1). This nano-LFIA was further integrated with "AdaptiScan", a smartphone-based detection system that uses adaptive detection algorithms to automatically extract and analyze fluorescence signals from LFIA strips. This integration of pore-size tailored DMSNs and "AdaptiScan" resulted in a limit of d

SUBMITTER: Ye S 

PROVIDER: S-EPMC11978842 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature