Femtosecond laser microfabrication of a fully-integrated optofluidic device for 3D imaging flow cytometry.

ABSTRACT: In recent years imaging flow cytometry (IFC) is gaining increasing attention as it combines the characteristics of conventional flow cytometry with optical microscopy techniques, allowing for high-throughput, multi-parameter screening of single cell populations. In the field of biology, the always increasing demand for high content morphological and spatial information led to the development of systems for volumetric imaging. However, current 3D IFC systems are often limited by the incompatibility with available microfluidic devices or by instrumental complexity that might lead to optical misalignment or mechanical instabilities in day-by-day operation. To this end, here we demonstrate the importance of advancing the laser fabrication technique by reporting on a fully integrated optofluidi

SUBMITTER: Sala F 

PROVIDER: S-EPMC11978988 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature