Transcriptional profiling of milk fat synthesis in mammary gland of dairy cows with different forage quality using RNA sequencing.

ABSTRACT:

Introduction

This study was designed to determine the effects of diets containing different-quality forages and concentrate content on milk composition and lipogenic enzyme expression in the mammary glands of dairy cows using RNA sequencing.

Methods

Thirty Holstein cows were randomly assigned to one of three treatments: mixed forage consisted of hay, silage, and alfalfa forage to a concentrate ratio of 54:46 (MF); corn stover as forage and forage to a concentrate ratio of 35:65 (CSA); corn stover as forage and forage to a concentrate ratio the same as MF (CSB). Mammary tissue biopsies were performed to analyze lipogenic enzyme expression using RNA-seq.

Results

Treatments did not affect dry matter intake, milk protein, or lactose. The milk yield, fat content and satura

SUBMITTER: Zhang H 

PROVIDER: S-EPMC11979108 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature