Anoctamin 9 determines Ca²⁺ signals during activation of T-lymphocytes.

ABSTRACT:

Background

Activation of T-cells is initiated by an increase in intracellular Ca²⁺, which underlies positive and negative regulation. Because the phospholipid scramblase and ion channel ANO9 (TMEM16J) was shown previously to regulated Ca²⁺ signals in renal epithelial cells, we asked whether ANO9 demonstrates a similar regulation in T-cells.

Methods

We used measurements of the intracellular Ca²⁺ concentration to examine the effects of ANO9 on intracellular Ca²⁺ signaling and demonstrated expression of ANO9 and its effects on cellular and molecular parameters.

Results

ANO9 was found to be expressed in human lymphocytes, including the Jurkat T-lymphocyte cell line and mouse lymphocytes. ANO9 has been shown to affect intracellul

SUBMITTER: Schreiber R 

PROVIDER: S-EPMC11979140 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature