CD40L stimulates tumor-infiltrating B-cells and improves ex vivo TIL expansion.

ABSTRACT:

Background

Adoptive transfer of tumor-infiltrating lymphocytes (TIL) is now a Food and Drug Administration (FDA)-approved treatment for melanoma. While this is a major milestone, there is room for improvement to increase clinical response rates and to further optimize the manufacturing of TIL products. In this study, we characterized the association of tumor-infiltrating B-cells (TIL-B) and tertiary lymphoid structures (TLSs) with clinical response to TIL therapy and tested whether the presence of B-cells in the tumor can be leveraged to optimize TIL manufacture.

Methods

Tumor sections from TIL responders (R, n=9) and non-responders (NR, n=11) were analyzed by RNA sequencing, and immune cell content was estimated in silico. To study the association between B-cells and TIL ex

SUBMITTER: Rossetti RAM 

PROVIDER: S-EPMC11979601 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature