Characterisation of macrophages in healthy and diseased livers in mice: identification of necrotic lesion-associated macrophages.

ABSTRACT:

Background

Healthy livers contain a large number of resident macrophages named Kupffer cells (KCs), which are partially replaced by infiltrating monocyte-derived macrophages (MoMFs) during acute or chronic liver injury. Despite extensive research, understanding macrophage heterogeneity, spatial distribution and interactions with other cells within the liver remains challenging.

Methods

This study employs sequential multiplex immunofluorescence staining, advanced image analysis and single-cell RNA sequencing (scRNA-seq) analysis to characterise macrophages in both healthy and diseased livers in mice.

Results

Our data revealed that liver KCs made up more than 80% of total immune cells in healthy mouse livers, while massive amounts of MoMFs infiltrated into the livers af

SUBMITTER: Feng D 

PROVIDER: S-EPMC11979608 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature