Identification of Candida auris and related species by multiplex PCR based on unique GPI protein-encoding genes.

ABSTRACT:

Background

The pathogen Candida auris is rapidly gaining clinical importance because of its resistance to antifungal treatments and its persistence in hospital environments. Early and accurate diagnosis of C. auris infections is crucial, and however, the fungus has often been misidentified by commercial systems.

Objectives

To develop conventional and real-time PCR methods for accurate and rapid identification of C. auris and its discrimination from closely related species by exploiting the uniqueness of certain glycosylphosphatidylinositol (GPI)-modified protein-encoding genes.

Methods

Species-specific primers for two unique putative GPI protein-encoding genes per species were designed for C. auris, C. haemulonii, C. pseudohaemulonii, C. duobushaemulonii, C. lusitania

SUBMITTER: Alvarado M 

PROVIDER: S-EPMC11979674 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature