Transcriptome profiling of maize transcription factor mutants to probe gene regulatory network predictions.

ABSTRACT: Transcription factors play important roles in regulation of gene expression and phenotype. A variety of approaches have been utilized to develop gene regulatory networks to predict the regulatory targets for each transcription factor, such as yeast-1-hybrid screens and gene co-expression network analysis. Here we identified potential transcription factor targets and used a reverse genetics approach to test the predictions of several gene regulatory networks in maize. Loss-of-function mutant alleles were isolated for 22 maize transcription factors. These mutants did not exhibit obvious morphological phenotypes. However, transcriptomic profiling identified differentially expressed genes in each of the mutant genotypes, and targeted metabolic profiling indicated variable phenolic accumulation

SUBMITTER: Ellison EL 

PROVIDER: S-EPMC11979765 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature