Development of a pod pepper vein yellows virus-based expression vector for the production of heterologous protein or virus like particles in Nicotiana benthamiana.

ABSTRACT: Plant viruses are emerging as a compelling alternative system for the heterologous production of pharmaceutical proteins, offering advantages in scalability, cost-effectiveness, and biological safety over traditional expression systems. They are increasingly recognized as effective platforms for biomedical applications, frequently used in the expression of human viral proteins and the display of peptides or proteins. The pod pepper vein yellows virus (PoPeVYV), classified within the genus Polerovirus of the Solemoviridae family, can substantially increase viral titers when co-infected with pod pepper vein yellows virus-associated RNA (PoPeVYVaRNA). This mixed infection methodology facilitates the formation of rod-shaped virus-like particles (VLPs), wherein modified green fluorescent protei

SUBMITTER: Wang L 

PROVIDER: S-EPMC11979914 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature