Characterization of HOL-30: a novel tandem-repeat galectin from the marine sponge <i>Halichondria okadai</i>.

ABSTRACT: We here report the novel primary structure of a new member in the galectin family, the β-galactoside-binding lectin HOL-30, from the marine sponge Halichondria okadai, whose full-length sequence was determined thanks to the combination between Edman degradation and transcriptome analysis. The HOL-30 polypeptide is a tandem-repeat dimeric galectin, consisting of 281 amino acids, which includes two carbohydrate recognition domains (CRDs). Unlike most other galectins described in Porifera, HOL-30 did not have a signal peptide sequence for secretion. In solution, HOL-30 exhibited a molecular weight of 60 kDa, indicating a dimeric organization consisting of two 30 kDa tandem-repeat subunits stabilized by non-covalent interactions. Although the two CRDs had a similar predicted 3D structur

SUBMITTER: Ohkawa M 

PROVIDER: S-EPMC11979922 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature