An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation.

ABSTRACT:

Objective

Understanding the regulation and function of plant genes is essential for addressing the challenges faced by modern agriculture. Plant transformation, in conjunction with fluorescence microscopy, offers a powerful approach to investigate the dynamic behavior of plant genes and the proteins they encode. We previously developed a set of Gateway-compatible tissue-specific plant transformation vectors. In this paper we aim to expand the toolkit of vectors available for Agrobacterium-mediated plant transformation and protoplast transfection.

Results

Here, we introduce new Agrobacterium-mediated plant transformation vectors by introducing additional fluorophores to create the pJRA vector series. Additionally, we introduce the pLCS series of vectors, a new set of modular

SUBMITTER: Allen JR 

PROVIDER: S-EPMC11980152 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature