The potential regulatory role of mannose phosphotransferase system EII in alkaline resistance of <i>Enterococcus faecalis</i>.

ABSTRACT:

Objective

To investigate the role of mannose phosphotransferase system (Man-PTS) EII in the alkaline resistance of Enterococcus faecalis (E. faecalis).

Methods

The Man-PTS EIID (mptD) deletion (ΔmptD) and overexpressing (+mptD) strains of E. faecalis were constructed. The contribution of the Man-PTS EII to alkaline resistance was investigated by assessing growth and biofilm formation of E. faecalis at pH 7 and 10, as well as changes in intracellular ATP levels, pH, potassium ion concentration, membrane potential and membrane permeability. The effects of mptD on the expression of other Man-PTS EII components and membrane transport-related genes were evaluated by RT-qPCR at pH 10. In addition, six clinical strains were is

SUBMITTER: Liu R 

PROVIDER: S-EPMC11980212 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature