Nanopore sequencing for precise detection of <i>Mycobacterium tuberculosis</i> and drug resistance: a retrospective multicenter study in China.

ABSTRACT: Tuberculosis (TB) management in endemic regions often grapples with resource constraints, including the scarcity of Mycobacterium tuberculosis (M.tb) culture laboratories. The emergence of M.tb strains with complex drug resistance profiles necessitates rapid and comprehensive drug susceptibility testing (DST) to guide patient treatment. However, traditional phenotypic DST (pDST) for M.tb is costly and time-consuming. In this study, we retrospectively enrolled 829 participants from six specialized TB treatment hospitals in China from September 2022 to July 2023. The diagnostic performance of TBseq test, a targeted-nanopore sequencing assay, was compared head-to-head with M.tb culture, acid-fast bacillus smear, quantitative polymerase chain reaction, and Xp

SUBMITTER: Yu S 

PROVIDER: S-EPMC11980377 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature