Long-read, multi-amplicon sequencing to explore genetic diversity associated with starch degrading phenotypes in amylolytic <i>Lactobacillaceae</i>.

ABSTRACT: Characterizing starch-degrading Lactobacillaceae and associated enzymes remains relevant as various industries seek to harness their activity to produce valuable by-products, develop novel food applications, and to aid the sustainable bioconversion of starch-rich resources. To support this, we developed a targeted methodological and analysis framework utilizing complimentary phenomic and genomic assays informative of the starch degrading potential of Lactobacillaceae. Adapted starch agar plate assays incorporating diversified starch sources and states facilitated the rating of extracellular amylolytic activity by starch-processing-line isolates [Lactobacillus amylovorus (n = 3), Lactobacillus amylolyticus (n = 2), and Limosilactobacillus reuteri

SUBMITTER: Olivier SA 

PROVIDER: S-EPMC11980422 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature