Investigating the relationships of structural and functional neural networks of primary visual cortex with engineered AAVs and chemogenetic-fMRI techniques.

ABSTRACT: Rationale: Neural networks are crucial for brain function, but the relationship between functional and structural networks remains unclear, hindering disease understanding and treatment. This study employs AAVs, MRI reporter gene (AQP1), and chemogenetic-fMRI to explore the relationship between structural and functional connectivity in the mouse primary visual cortex (V1), offering a novel approach to study abnormal brain functions. Methods: The rAAV-PHP.eB-DIO vector encoding AQP1 was used to cross the blood-brain barrier and infect the brain, enabling in vivo diffusion-weighted imaging (DWI-MRI) to assess the anterograde structural connectivity network of V1. Additionally, chemogenetic activation of V1 using a Cre-dependent system was performed, and the whole-brain B

SUBMITTER: Gui Z 

PROVIDER: S-EPMC11980648 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature