Designing multifunctional recombinant vaccines: an engineering strategy based on innovative epitope prediction-guided splicing.

ABSTRACT: Background: Recombinant subunit vaccines leveraging pathogen-derived components are pivotal for disease prevention. Nonetheless, application of these vaccines still faces challenges such as low immunogenicity and a short half-life. Additionally, selecting appropriate antigens presents a significant barrier in recombinant vaccine design. Methods: Here, we applied a novel approach to address these challenges by developing recombinant vaccines targeting LMP2A. We employed in silico epitope prediction and splicing to create epitope enrichment regions (EERs) in conjunction with the TLR4 agonist hEDA to enhance immunogenicity and the immunoglobulin G1 (IgG1) Fc fragment to prolong persistence. Results: This multifaceted strategy enhances antigen uptake by antigen-pres

SUBMITTER: Wang Z 

PROVIDER: S-EPMC11980677 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature