COPB1 deficiency triggers osteoporosis with elevated iron stores by inducing osteoblast ferroptosis.

ABSTRACT:

Background

Osteoporosis (OP) is a systemic bone metabolic disease that results from an imbalance between bone formation and bone resorption. The accumulation of iron has been identified as an independent risk factor for osteoporosis. Ferroptosis, a novel form of programmed cell death, is driven by iron-dependent lipid peroxidation. Nevertheless, the precise role of ferroptosis in iron accumulation-induced osteoporosis remains uncertain.

Methods

We utilized proteomics and ELISA to screen key regulatory molecules related to iron accumulation in osteoporosis populations. HE staining was used to assess osteocyte changes in Hamp knockout (KO) iron accumulation mouse models. Western Blot, qPCR, ALP staining, and Alizarin Red staining were employed to explore the effects of siRNA-m

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11981772 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature