Identification of protein-degraders in an anaerobic digester by protein stable isotope probing and metagenomics.

ABSTRACT: Presence of carbohydrates hampers protein degradation in anaerobic digesters. To understand this phenomenon, we used proteogenomics to identify the active protein-degraders in the presence of low and high carbohydrates concentrations. Active metabolic pathways of the identified protein-degraders were investigated using proteomics with ¹³C-protein substrates (protein stable isotope probing). Results showed that 1) Acinetobacter was the active protein-degraders under both protein-fed and protein-glucose mixture-fed conditions, 2) the relative abundance of Acinetobacter was not affected by the presence of carbohydrates, 3) the incorporation of the ¹³C-labelled protein substrate was predominantly observed in outer membrane-bound proteins and porin proteins, which are asso

SUBMITTER: Deng Z 

PROVIDER: S-EPMC11982144 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature