Unravelling the conundrum of nucleolar NR2F1 localization using antibody-based approaches in vitro and in vivo.

ABSTRACT: As a transcription factor, NR2F1 regulates spatiotemporal gene expression in the nucleus particularly during development. Aberrant NR2F1 causes the rare neurodevelopmental disorder Bosch-Boonstra-Schaaf Optic Atrophy Syndrome. In addition, altered NR2F1 expression is frequently observed in various cancers and is considered a prognostic marker or potential therapeutic target. NR2F1 has been found in both the nucleus and nucleoli, suggesting a non-canonical and direct role in the latter compartment. Hence, we studied this phenomenon employing various in vitro and in vivo models using different antibody-dependent approaches. Examination of seven commonly used anti-NR2F1 antibodies in different human cancer and stem cells as well as in wild type and null mice revealed that NR2F1 nucleolar loca

SUBMITTER: Bertacchi M 

PROVIDER: S-EPMC11982218 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature