AAV-mediated expression of proneural factors stimulates neurogenesis from adult Muller glia in vivo.

ABSTRACT: The lack of regeneration in the human central nervous system (CNS) has major health implications. To address this, we previously used transgenic mouse models to show that neurogenesis can be stimulated in the adult mammalian retina by driving regeneration programs that other species activate following injury. Expression of specific proneural factors in adult Müller glia causes them to re-enter the cell cycle and give rise to new neurons following retinal injury. To bring this strategy closer to clinical application, we now show that neurogenesis can also be stimulated when delivering these transcription factors to Müller glia using adeno-associated viral (AAV) vectors. AAV-mediated neurogenesis phenocopies the neurogenesis we observed from transgenic animals, with different proneural facto

SUBMITTER: Pavlou M 

PROVIDER: S-EPMC11982270 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature