Testis-enriched <i>Spsb1</i> is not required for spermatogenesis and fertility in mice.

ABSTRACT:

Objectives

SPRY (repeats in splA and RyR) domain-containing SOCS (suppressor of cytokine signaling) box protein 1 (SPSB1) is an E3 ligase adaptor protein that has been implicated in various cellular processes and physiological pathways. However, its role in spermatogenesis remains poorly understood. The objective of this study was to investigate the impact of SPSB1 deficiency on spermatogenesis and male fertility in mice.

Methods

We generated Spsb1 knockout (Spsb1-KO) mice to explore the effects of SPSB1 deficiency on sperm quality. To assess sperm parameters, we utilized computer-assisted sperm analysis (CASA), which provides precise measurements of sperm motility, concentration, and morphology. Additionally, histological and immunohistochemical analyses were

SUBMITTER: Hu H 

PROVIDER: S-EPMC11982841 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature