Decoding Specificity of Cyanobacterial MysDs in Mycosporine-like Amino Acid Biosynthesis through Heterologous Expression in <i>Saccharomyces cerevisiae</i>.

ABSTRACT: Mycosporine-like amino acids (MAAs) are potent natural UV-protectants, but their industrial production is hindered by efficiency and sustainability issues in large-scale extraction of native hosts. Heterologous biosynthesis in chassis organisms provides a promising alternative route, although the substrate promiscuity of the ATP-grasp ligase MysD complicates the biosynthesis of specific MAAs. In this study, we developed a Saccharomyces cerevisiae strain with enhanced capacity of producing mycosporine-glycine (MG). This strain serves as an efficient MysD expression platform that converts MG into shinorine and porphyra-334. Through structural modeling, site-directed mutagenesis, and mutant characterization, we identified two residues on the omega-loop of MysD involved in determining p

SUBMITTER: Zheng X 

PROVIDER: S-EPMC11983340 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature