Rapid Diagnostic Sequencing of Stool DNA Using Targeted Nanopore Sequencing in Patients With a Pulmonary Tuberculosis Diagnosis.

ABSTRACT:

Background

Approximately 1.25 million individuals died of tuberculosis in 2023, in part due to ineffective treatment. In patients with paucibacillary tuberculosis, microbiologic confirmation and drug resistance testing via respiratory specimens is challenging; hence, stool samples are increasingly used for microbiologic confirmation. Targeted next-generation sequencing (tNGS) of stool DNA may improve detection of drug-resistant (DR) tuberculosis, helping patients receive appropriate treatments.

Methods

We assessed the ability of a nanopore tNGS approach using stool to detect drug resistance in a prospective, nested cohort of consecutive participants in Eswatini with pulmonary tuberculosis confirmed via sputum culture or sputum GeneXpert Ultra from 2020 to 2023. We compared s

SUBMITTER: Ness T 

PROVIDER: S-EPMC11983389 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature