An AIE-active probe acts as novel tool for rapid and accurate quantitative antifungal susceptibility assessment.

ABSTRACT:

Objectives

Common antifungal susceptibility testing methods are often time-consuming and subject to interpretation bias in endpoint determination, making them inadequate for clinical applications. We aim to develop a rapid and accurate quantitative method for routine antifungal susceptibility testing in diagnostic laboratories by employing the aggregation-induced emission (AIE) luminogen TBP-2.

Methods

The AIE luminogen TBP-2 with two positive charges was introduced to develop an antifungal susceptibility testing protocol based on the reference broth microdilution (BMD) method. The minimum inhibitory concentration of different drugs against Candida was determined by detecting changes in fluorescence intensity. A total of 76 clinical isolates of Candida albicans

SUBMITTER: Zheng F 

PROVIDER: S-EPMC11983505 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature