Reduction of alternative polarization of macrophages by short-term activated hepatic stellate cell-derived small extracellular vesicles.

ABSTRACT:

Background

Activated hepatic stellate cells (HSCs) induce alternative (M2) polarization of macrophages and contribute to the progression of fibrosis and hepatocellular carcinoma (HCC). However, the effects of small extracellular vesicles released by HSCs (HSC-sEVs) during activation remain largely unknown.

Methods

The aim of this study was to investigate the role of extracellular vesicles released by HSCs (HSC-sEVs) at different stages of activation in macrophage polarization. The effects of sEVs from short-term activated and long-term activated HSCs on liver macrophages was studied. Small RNA sequencing analyses were performed to obtain differential miRNAs transported by the short-term and long-term activated HSC- sEVs. The in vivo effects of short-term activated HSC-sEV-sp

SUBMITTER: Sun Y 

PROVIDER: S-EPMC11983935 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature