Complete Enzymatic Depolymerization of Polyethylene Terephthalate (PET) Plastic Using a <i>Saccharomyces cerevisiae-</i>Based Whole-Cell Biocatalyst.

ABSTRACT: Management of polyethylene terephthalate (PET) plastic waste remains a challenge. PET-hydrolyzing enzymes (PHEs) such as IsPETase and variants like FAST-PETase demonstrate promising PET depolymerization capabilities at ambient temperatures and can be utilized to recycle and upcycle plastic waste. Whole-cell biocatalysts displaying PHEs on their surface offer high efficiency, reusability, and stability for PET depolymerization. However, their efficacy in fully breaking down PET is hindered by the necessity of two enzymes: PETase and MHETase. Current whole-cell systems either display only one enzyme or struggle with performance when displaying larger enzymes such as the MHETase-PETase chimera. We developed a Saccharomyces cerevisiae-based whole-cell biocatalyst for complete dep

SUBMITTER: Gulati S 

PROVIDER: S-EPMC11984091 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature