Development of an RT-RPA assay for La Crosse virus detection provides insights into age-dependent neuroinvasion in mice.

ABSTRACT:

Background

La Crosse virus (LACV) is a mosquito-borne arbovirus responsible for pediatric encephalitis in North America, predominantly affecting children under 16 years. Early and accurate diagnosis is critical to reducing morbidity in this vulnerable population. However, existing molecular and serological methods are limited in sensitivity, specificity, and accessibility.

Methods

To address these limitations, we developed a reverse transcription recombinase polymerase amplification (RT-RPA) assay for LACV detection. Primers targeting the divergent M segment of the LACV genome were designed and screened for optimal performance. The assay's analytical sensitivity was evaluated through serial dilutions of LACV RNA prior to reverse transcription, while specificity was assessed

SUBMITTER: Lumkong L 

PROVIDER: S-EPMC11984161 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature