Increased amounts of cell-free DNA released from a culture with a high content of cancer stem cells.

ABSTRACT:

Background

The study and characterization of cell-free DNA (cfDNA) has gained significant importance due to its clinical applications as a diagnostic and prognostic marker. However, it remains unclear whether all cell populations within a tumor or culture contribute equally to its release. This pioneering research analyzes the contribution of cancer stem cells (CSCs) in colon cancer cell lines to the amount of cfDNA released and its role in cellular transformation.

Methods

The CSC population derived from the SW480 colon cancer cell line was enriched using a non-adhesive culture system to assess the quantity and electrophoretic profile of the released cfDNA. Subsequently, in vitro transformation assays were conducted to compare the transforming capacity of the cfDNA ob

SUBMITTER: Fernandez-Dominguez IJ 

PROVIDER: S-EPMC11985834 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature