Revealing the role of the <i>AGO4</i> gene against rice <i>hoja blanca</i> virus: from transformation to protein structure.

ABSTRACT: Rice hoja blanca virus (RHBV), transmitted by the insect vector Tagosodes orizicolus, poses a significant threat to rice cultivation. Here, we use CRISPR/Cas9 technology to produce specific mutations in the AGO4 gene of Oryza sativa, using the Fedearroz 2000 variety, with the aim of elucidating the participation of the gene in resistance to RHBV. We obtained 14 edited plants that presented with deletions of one, two, and three nucleotides in the sequence of exon 23 of the AGO4 gene. Phenotypic evaluations showed an increase in susceptibility to RHBV in the edited lines. We identified the presence of RHBV in the leaf tissue of infected plants by amplifying the nucleoprotein, NS3, and NS4 genes of the virus. Using RT-qPCR, we analyzed the expr

SUBMITTER: Nanez J 

PROVIDER: S-EPMC11985844 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature